Composite

Part:BBa_K2922044:Design

Designed by: Yuan Li   Group: iGEM19_XMU-China   (2019-10-14)


Co-expression system of BBa_K346030 and BBa_K2922042 for enhancing lead absorption


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 520
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 308
    Illegal XhoI site found at 1005
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 520
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 520
    Illegal AgeI site found at 140
  • 1000
    COMPATIBLE WITH RFC[1000]


Design notes

Construct from existed parts according to Standard BioBrick Assembly


Source

Cav1 is from homo sapiens. DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.


Reference

1. J. Shin et al., Display of membrane proteins on the heterologous caveolae carved by caveolin-1 in the Escherichia coli cytoplasm. Enzyme Microb Technol 79-80, 55-62 (2015).
2. J. Shin et al., Endocytosing Escherichia coli as a Whole-Cell Biocatalyst of Fatty Acids. ACS Synthetic Biology 8, 1055-1066 (2019).